December 12, 2020
Differences in Gut Microbiome in Hospitalized Immunocompetent vs. Immunocompromised Children, Including Those With Sickle Cell Disease
Background: Intestine microbial range and composition play vital roles in well being. This cross-sectional research was designed to take a look at the speculation that hospitalized youngsters who could also be comparatively immunocompromised (IC), outlined as these with most cancers, sickle cell illness (SCD), transplantation, or receiving immunosuppressive remedy) would have decreased microbial range, elevated Clostridioides difficile colonization and totally different species composition in comparison with non-immunocompromised (Non-IC) youngsters admitted to the identical pediatric unit.
Strategies: A stool pattern was obtained inside 72 h of admission to a single unit at The Kids’s Hospital at Montefiore, Bronx, NY from March 2016 to February 2017 and the microbiome assessed by 16S rRNA sequencing. C. difficile colonization was assessed by glutamate dehydrogenase antigen and toxin polymerase chain response assays.
Outcomes: Stool samples have been obtained from 69 IC (32 SCD, 19 most cancers, 9 transplantation and 9 different) and 37 Non-IC sufferers. There have been no vital variations in microbial alpha range and C. difficile colonization evaluating IC vs. non-IC sufferers. Decrease alpha range, nevertheless, was independently related to the usage of proton pump inhibitors or antibiotics, together with prophylactic penicillin in sufferers with SCD. Variations in particular species abundances have been noticed when evaluating IC vs. non-IC sufferers, significantly youngsters with SCD. Non-IC sufferers had elevated abundance of commensals related to well being together with Alistipes putredinis, Alistipes ihumii, Roseburia inulinivorans, Roseburia intake a look atinalis, and Ruminococcus albus (p < 0.005).
Conclusions: Antibiotics and proton pump inhibitors, which have been extra generally utilized in IC youngsters, have been recognized as danger components for decrease microbial range. Non-IC sufferers had greater abundance of a number of bacterial species related to well being. Longitudinal research are wanted to find out the scientific significance of those variations in intestine microbiome.
Mixture of Silibinin and Curcumin Diminished Leptin Receptor Expression in MCF-7 Human Breast Most cancers Cell Line
Background: Leptin and leptin receptor (Ob-R) are related to worse prognosis, distant metastasis, and poor survival of breast most cancers. We investigated the cytotoxic impact of silibinin and curcumin, individually and mixed, on Ob-R expression in MCF-7 cells.
Strategies: This research was carried out from October 2017 to April 2018 on the Division of Medical Biochemistry, College of Drugs, Ahvaz Jundishapur College of Medical Sciences, Ahvaz, Iran. The cytotoxic impact of silibinin and curcumin, individually and mixed, and their corresponding half-maximal inhibitory focus (IC50) values have been decided utilizing the methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay. The cells have been handled with totally different concentrations of silibinin (50-400 μM), curcumin (10-35 μM), and their combos for 24 and 48 hours. The expression of Ob-R was measured utilizing the Western blot evaluation by treating the cells with totally different concentrations of curcumin (10-25 μM), silibinin (50-250 μM), and their respective combos. The distinction in imply cell viability between the teams was calculated utilizing one-way ANOVA adopted by Tukey’s put up hoc take a look at.
Outcomes: Silibinin and curcumin exerted time- and dose-dependent cytotoxic impact on MCF-7 cells. After therapy with silibinin, the IC50 values have been about 250 and 50 μM at 24 and 48 hours, respectively. When it comes to therapy with curcumin, the IC50 values have been about 25 and 15 μM at 24 and 48 hours, respectively. Following therapy with silibinin, the Western blot evaluation confirmed that Ob-R expression considerably decreased at 150 μM (P=0.031) and 200 μM (P=0.023) concentrations. Curcumin didn’t considerably lower the Ob-R expression, nevertheless, the expression considerably decreased (P=0.004) when it was mixed with silibinin.
Conclusion: The mix of silibinin and curcumin considerably decreased Ob-R expression in MCF-7 cells in contrast with their particular person results.
Differences in Gut Microbiome in Hospitalized Immunocompetent vs. Immunocompromised Children, Including Those With Sickle Cell Disease
Low frequency deep mind stimulation within the inferior colliculus ameliorates haloperidol-induced catalepsy and reduces nervousness in rats
Deep mind stimulation (DBS) of the colliculus inferior (IC) improves haloperidol-induced catalepsy and induces paradoxal kinesia in rats. For the reason that IC is a part of the mind aversive system, DBS of this construction has lengthy been associated to aversive conduct in rats limiting its scientific use. This research aimed to enhance intracollicular DBS parameters so as to keep away from anxiogenic unwanted side effects whereas preserving motor enhancements in rats. Catalepsy was induced by systemic haloperidol (0.5mg/kg) and after 60 min the bar take a look at was carried out throughout which a given rat obtained steady (5 min, with or with out pre-stimulation) or intermittent (5 x 1 min) DBS (30Hz, 200-600μA, pulse width 100μs). Solely steady DBS with pre-stimulation decreased catalepsy time. The rats have been additionally submitted to the elevated plus maze (EPM) take a look at and obtained both steady stimulation with or with out pre-stimulation, or sham therapy.
Solely rats receiving steady DBS with pre-stimulation elevated the time spent and the variety of entries into the open arms of the EPM suggesting an anxiolytic impact. The current intracollicular DBS parameters induced motor enhancements with none proof of aversive conduct, pointing to the IC in its place DBS goal to induce paradoxical kinesia bettering motor deficits in parkinsonian sufferers. Q-tip take a look at affords a easy strategy for figuring out urethral hypermobility. Contemplating surgical therapy, stress urinary incontinence (SUI) should be categorised and the contribution of intrinsic sphincter deficiency (ISD) and/or urethral hypermobility should be decide.
THIOGLYCOLLATE MEDIUM, BREWER |
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THIOGLYCOLLATE MEDIUM, BREWER |
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SODIUM THIOGLYCOLLATE |
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SODIUM THIOGLYCOLLATE |
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SODIUM THIOGLYCOLLATE |
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Thioglycollate U.S.P. |
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| MED1126 | Scientific Laboratory Supplies | PK50 | EUR 45.6 |
ALT. THIOGLYCOLLATE MED. |
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ALT. THIOGLYCOLLATE MED. |
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ALT. THIOGLYCOLLATE MED. |
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CASEIN ENRICHED PEPTONE |
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CASEIN ENRICHED PEPTONE |
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CASEIN ENRICHED PEPTONE |
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| C03-105-500g | Alphabiosciences | 500 g | EUR 134.4 |
Rabbit IgG Enriched | RA-15G |
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| RA-15G | Immunology Consultants Laboratory | 20 mg | EUR 145 |
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Description: Rabbit IgG Enriched | RA-15G | Immunology Consultants Laboratory Species Reactivity: Rabbit Format: Unconjugated AP Product Type: Isotype Control Antibody clonality: Polyclonal |
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Cat IgG Enriched | RA-20G |
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| RA-20G | Immunology Consultants Laboratory | 20 mg | EUR 289 |
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Description: Cat IgG Enriched | RA-20G | Immunology Consultants Laboratory Species Reactivity: Cat Format: Unconjugated AP Product Type: Isotype Control Antibody clonality: Polyclonal |
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Rat IgG Enriched | RA-25G |
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| RA-25G | Immunology Consultants Laboratory | 20 mg | EUR 274 |
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Description: Rat IgG Enriched | RA-25G | Immunology Consultants Laboratory Species Reactivity: Rat Format: Unconjugated AP Product Type: Isotype Control Antibody clonality: Polyclonal |
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Chicken IgY Enriched | RA-30Y |
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| RA-30Y | Immunology Consultants Laboratory | 25 mg | EUR 243 |
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Description: Chicken IgY Enriched | RA-30Y | Immunology Consultants Laboratory Species Reactivity: Chicken Format: Unconjugated AP Product Type: Isotype Control Antibody clonality: Polyclonal |
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Dog IgG Enriched | RA-40G |
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| RA-40G | Immunology Consultants Laboratory | 20 mg | EUR 274 |
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Description: Dog IgG Enriched | RA-40G | Immunology Consultants Laboratory Species Reactivity: Dog Format: Unconjugated AP Product Type: Isotype Control Antibody clonality: Polyclonal |
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Mouse IgG Enriched | RA-90G |
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| RA-90G | Immunology Consultants Laboratory | 20 mg | EUR 259 |
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Description: Mouse IgG Enriched | RA-90G | Immunology Consultants Laboratory Species Reactivity: Mouse Format: Unconjugated AP Product Type: Isotype Control Antibody clonality: Polyclonal |
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A Medium |
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| DJ1018 | Bio Basic | 100g | EUR 101.76 |
DC MEDIUM |
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| D04-117-10kg | Alphabiosciences | 10 kg | EUR 1894.8 |
DC MEDIUM |
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DC MEDIUM |
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| D04-117-500g | Alphabiosciences | 500 g | EUR 168 |
Advanced Medium |
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| C0003-04 | Addexbio | RT 500 mL Bottle | EUR 123.6 |
Medium 199 |
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| C0012-01 | Addexbio | RT 500 mL Bottle | EUR 126 |
COLIFORM MEDIUM |
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COLIFORM MEDIUM |
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COLIFORM MEDIUM |
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Heller's Medium |
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| CP014-010 | GenDepot | 10X1L | EUR 118.8 |
Heller's Medium |
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| CP014-500 | GenDepot | 50L | EUR 151.2 |
Hoagland's Medium |
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| CP015-010 | GenDepot | 10X1L | EUR 118.8 |
Hoagland's Medium |
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| CP015-500 | GenDepot | 50L | EUR 151.2 |
EC MEDIUM |
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| E05-100-10kg | Alphabiosciences | 10 kg | EUR 976.8 |
EC MEDIUM |
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EC MEDIUM |
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Mounting Medium |
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| 4300 | Virostat | 3 ml | EUR 216.6 |
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Description: This is Mounting medium (non-fading) used for maintaining optimal conditions needed to obtain the maximum fluorescence emission from Fluorescein. |
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NeuroProgenitor Medium |
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| NM42400 | Neuromics | 125 ml | EUR 364.8 |
HLP MEDIUM |
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HLP MEDIUM |
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| H08-107-2Kg | Alphabiosciences | 2 Kg | EUR 640.8 |
HLP MEDIUM |
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SIM MEDIUM |
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SIM MEDIUM |
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| S19-110-2kg | Alphabiosciences | 2kg | EUR 313.2 |
SIM MEDIUM |
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SOB MEDIUM |
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| S19-124-10kg | Alphabiosciences | 10 kg | EUR 1158 |
SOB MEDIUM |
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| S19-124-2kg | Alphabiosciences | 2kg | EUR 298.8 |
SOB MEDIUM |
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| S19-124-500g | Alphabiosciences | 500 g | EUR 124.8 |
M9 Medium |
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| SD7024 | Bio Basic | 250g | EUR 86.1 |
M9CA Medium |
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| SD7025 | Bio Basic | 250g | EUR 86.1 |
YM Medium |
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| SD7031 | Bio Basic | 250g | EUR 84.54 |
TYGPN Medium |
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| SD7032 | Bio Basic | 250g | EUR 84.01 |
M63 Medium |
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| SD7033 | Bio Basic | 250g | EUR 86.1 |
TCBS Medium |
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| MED1188 | Scientific Laboratory Supplies | PK10 | EUR 7.52 |
DNase Medium |
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| MED1196 | Scientific Laboratory Supplies | PK10 | EUR 7.98 |
OF Medium |
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| MED1664 | Scientific Laboratory Supplies | 500G | EUR 68.4 |
Phosphoprotein Enriched in Astrocytes 15 Protein |
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| 20-abx262863 | Abbexa |
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Insect Cell Medium: TNM-FH Insect Culture Medium |
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| ABP-MED-10001 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Serum-Free Insect Culture Medium |
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| ABP-MED-10002 | Allele Biotech | 1 liter | Ask for price |
Insect Cell Medium: Grace?s Insect Medium (Unsupplemented) |
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| ABP-MED-10004 | Allele Biotech | 1 liter | Ask for price |
Recombinant Human Phosphoprotein Enriched in Astrocytes 15 |
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| 7-05845 | CHI Scientific | 2µg | Ask for price |
Recombinant Human Phosphoprotein Enriched in Astrocytes 15 |
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| 7-05846 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Phosphoprotein Enriched in Astrocytes 15 |
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| 7-05847 | CHI Scientific | 1mg | Ask for price |
Recombinant Human Ras Homolog Enriched in Brain |
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| 7-06136 | CHI Scientific | 10µg | Ask for price |
Recombinant Human Ras Homolog Enriched in Brain |
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| 7-06137 | CHI Scientific | 50µg | Ask for price |
Recombinant Human Ras Homolog Enriched in Brain |
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| 7-06138 | CHI Scientific | 1mg | Ask for price |
Ras Homolog Enriched In Brain (RHEB) Antibody |
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| abx145070-100ug | Abbexa | 100 ug | EUR 469.2 |
Ras Homolog Enriched In Brain (RHEB) Antibody |
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| 20-abx142196 | Abbexa |
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Proteolipid Protein 2 (Colonic Epithelium-Enriched) Antibody |
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| 20-abx114890 | Abbexa |
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Ras Homolog Enriched In Brain (RHEB) Antibody |
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| 20-abx115059 | Abbexa |
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Ras Homolog Enriched In Brain (RHEB) Antibody |
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| 20-abx001079 | Abbexa |
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Ras Homolog Enriched In Brain (RHEB) Antibody |
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| abx026430-400ul | Abbexa | 400 ul | EUR 627.6 |
Ras Homolog Enriched In Brain (RHEB) Antibody |
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| abx026430-80l | Abbexa | 80 µl | EUR 343.2 |
Ras Homolog Enriched In Brain (RHEB) Protein |
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| 20-abx260430 | Abbexa |
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Pseudopodium-enriched atypical kinase 1 (PEAK1) Antibody |
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| abx331275-100ul | Abbexa | 100 ul | EUR 510 |
Pseudopodium-enriched atypical kinase 1 (PEAK1) Antibody |
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| 20-abx324093 | Abbexa |
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Ras Homolog Enriched In Brain (RHEB) Antibody |
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| 20-abx313390 | Abbexa |
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Ras Homolog Enriched In Brain (RHEB) Antibody |
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| 20-abx242233 | Abbexa |
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Ras Homolog Enriched In Brain (RHEB) Antibody |
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| 20-abx214959 | Abbexa |
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Ras Homolog Enriched In Brain (RHEB) Antibody |
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| 20-abx214960 | Abbexa |
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Ras Homolog Enriched In Brain (RHEB) Antibody |
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| 20-abx213656 | Abbexa |
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Ras Homolog Enriched In Brain (RHEB) Antibody |
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| 20-abx213657 | Abbexa |
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Ras Homolog Enriched In Brain (RHEB) Antibody |
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| 20-abx214387 | Abbexa |
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Ras Homolog Enriched In Brain (RHEB) Antibody |
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| abx237280-100ug | Abbexa | 100 ug | EUR 610.8 |
MCDB 131 Medium |
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| CM034-050 | GenDepot | 500ml | EUR 102 |
MCDB 131 Medium |
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| CM034-300 | GenDepot | 6x500ml | EUR 198 |
MCDB 131 Medium |
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| CM034-310 | GenDepot | 10x500ml | EUR 262.8 |
MCDB 131 Medium |
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| CM034-320 | GenDepot | 20x500ml | EUR 408 |
MCDB 131 Medium |
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| CM034-350 | GenDepot | 50x500ml | EUR 702 |
Williams' Medium E |
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| CM063-050 | GenDepot | 500ml | EUR 109.2 |
Williams' Medium E |
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| CM063-300 | GenDepot | 6x500ml | EUR 319.2 |
Williams' Medium E |
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| CM063-310 | GenDepot | 10x500ml | EUR 375.6 |
Williams' Medium E |
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| CM063-320 | GenDepot | 20x500ml | EUR 724.8 |
Williams' Medium E |
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| CM063-350 | GenDepot | 50x500ml | EUR 1170 |
H Medium Broth |
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| DJ1019 | Bio Basic | 100g | EUR 73.06 |
AddexBio Cryopreservation Medium |
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| C0002-20mL | Addexbio | RT vial | EUR 124.8 |
CARY & BLAIR MEDIUM |
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| C03-131-10kg | Alphabiosciences | 10 kg | EUR 2622 |
CARY & BLAIR MEDIUM |
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| C03-131-2Kg | Alphabiosciences | 2 Kg | EUR 616.8 |
CARY & BLAIR MEDIUM |
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| C03-131-500g | Alphabiosciences | 500 g | EUR 211.2 |
Cereal Crops Medium |
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| CP003-005 | GenDepot | 5L | EUR 135.6 |
Cereal Crops Medium |
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| CP003-010 | GenDepot | 10X1L | EUR 202.8 |
Kao Michayluk Medium |
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| CP017-010 | GenDepot | 10X1L | EUR 118.8 |
Kao Michayluk Medium |
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| CP017-500 | GenDepot | 50L | EUR 151.2 |
Orchid Maintenance Medium |
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| CP037-010 | GenDepot | 10X1L | EUR 130.8 |
Orchid Maintenance Medium |
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| CP037-500 | GenDepot | 50L | EUR 165.6 |
Woody Plants Medium |
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| CP040-010 | GenDepot | 10X1L | EUR 121.2 |
Woody Plants Medium |
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| CP040-500 | GenDepot | 50L | EUR 151.2 |
EC MEDIUM, MODIFIED |
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| E05-108-10kg | Alphabiosciences | 10 kg | EUR 1029.6 |
EC MEDIUM, MODIFIED |
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| E05-108-2Kg | Alphabiosciences | 2 Kg | EUR 271.2 |
EC MEDIUM, MODIFIED |
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| E05-108-500g | Alphabiosciences | 500 g | EUR 117.6 |
FluoreGuard Mounting Medium |
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| FMM030 | ScyTek Laboratories | 30 ml | EUR 115.2 |
FluoreGuard Mounting Medium |
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| FMM060 | ScyTek Laboratories | 60 ml | EUR 139.2 |
FluoreGuard Mounting Medium |
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| FMM500 | ScyTek Laboratories | 500 ml | EUR 392.4 |
FluoreGuard Mounting Medium |
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| FMM999 | ScyTek Laboratories | 1000 ml | EUR 636 |
Recovery Medium - 6x12mL |
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| 1711 | Intact Genomics | 4/PK | EUR 153.6 |
A-1 MEDIUM |
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| A01-116-10kg | Alphabiosciences | 10 kg | EUR 1240.8 |
A-1 MEDIUM |
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| A01-116-2Kg | Alphabiosciences | 2 Kg | EUR 316.8 |
A-1 MEDIUM |
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| A01-116-500g | Alphabiosciences | 500 g | EUR 129.6 |
Aqueous Mounting Medium |
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| AR1018 | BosterBio | 10mL X5 (Enough for 800-1200 assays) | EUR 96 |
Antifade Mounting Medium |
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| AR1109 | BosterBio | 10mL (for 1000 assays) | EUR 78 |
MOTILITY TEST MEDIUM |
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| M13-121-10kg | Alphabiosciences | 10 kg | EUR 1833.6 |
MOTILITY TEST MEDIUM |
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MOTILITY TEST MEDIUM |
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OF BASAL MEDIUM |
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| O15-100-10kg | Alphabiosciences | 10 kg | Ask for price |
OF BASAL MEDIUM |
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OF BASAL MEDIUM |
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| O15-100-500g | Alphabiosciences | 500 g | Ask for price |
Hepatocyte Maintenance Medium |
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| HM42600 | Neuromics | 100 ml | EUR 490.8 |
Neuron Growth Medium |
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| HNM001 | Neuromics | 500 ml | EUR 397.2 |
Agarose, medium EEO |
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| GE4750-100G | Glentham Life Sciences | 100 g | EUR 199.2 |
Agarose, medium EEO |
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| GE4750-500G | Glentham Life Sciences | 500 g | EUR 514.8 |
REINFORCED CLOSTRIDIUM MEDIUM |
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| R18-105-10kg | Alphabiosciences | 10 kg | EUR 1432.8 |
REINFORCED CLOSTRIDIUM MEDIUM |
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| R18-105-2kg | Alphabiosciences | 2kg | EUR 358.8 |
REINFORCED CLOSTRIDIUM MEDIUM |
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| R18-105-500g | Alphabiosciences | 500 g | EUR 141.6 |
Schwann Growth Medium |
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| SGM001 | Neuromics | 500 ml | EUR 292.8 |
Sepadextran-25 Medium |
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| SD25M-100 | ABTBeads | 100 g | EUR 325.2 |
Sepadextran-25 Medium |
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| SD25M-500 | ABTBeads | 500 g | EUR 1243.2 |
Sepadextran-50 Medium |
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| SD50M-100 | ABTBeads | 100 g | EUR 412.8 |
Sepadextran-50 Medium |
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| SD50M-500 | ABTBeads | 500 g | EUR 1542 |
TERRIFIC BROTH MEDIUM |
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| T20-114-10kg | Alphabiosciences | 10 kg | EUR 810 |
TERRIFIC BROTH MEDIUM |
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| T20-114-2kg | Alphabiosciences | 2kg | EUR 223.2 |
TERRIFIC BROTH MEDIUM |
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| T20-114-500g | Alphabiosciences | 500 g | EUR 104.4 |
2XYT MICROBIAL MEDIUM |
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| X24-102-10kg | Alphabiosciences | 10 kg | EUR 736.8 |
2XYT MICROBIAL MEDIUM |
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| X24-102-2Kg | Alphabiosciences | 2 Kg | EUR 207.6 |
2XYT MICROBIAL MEDIUM |
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| X24-102-500g | Alphabiosciences | 500 g | EUR 99.6 |
Lowenstein-Jensen Medium |
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| MED1400 | Scientific Laboratory Supplies | EACH | EUR 114 |
Sabouraud Liquid Medium |
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| MED1462 | Scientific Laboratory Supplies | EACH | EUR 54.61 |
Slanetz & Bartley Medium |
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| MED1468 | Scientific Laboratory Supplies | EACH | EUR 107.16 |
We consider there is a correlation between stomach leak level stress (ALPP) and urethral mobility diploma, and the goal of this research is to discover it utilizing Q-tip. We carried out a potential research, between years 2014 and 2016. Females over 18 years presenting with indicators and signs of SUI in line with the 2002 ICS Standardization of Terminology have been included. Evaluation was made with the Worldwide Session on Incontinence Questionnaire Quick Kind (ICIQ-SF), the Q-tip take a look at and invasive urodynamics.
