July 13, 2021

FastAmp Direct PCR

By Alejandra

We invented a truly innovative product in the FastAmp Plant Direct PCR Kit, Genotyping kit, and Plant Direct PCR & Genotyping Solution. The FastAmp line of products allows for easy and robust amplification of DNA fragments from plant tissues with high specificity and high sensitivity without the need for complicated DNA purification steps. The advanced formulation of these products also allows fast PCR cycling conditions without compromising PCR sensitivity, specificity, and yield. FastAmp Plant Direct PCR is ideal and powerful for high-throughput genotyping, DNA amplification, and plant genome analysis.

Benefits:

  • Save Time — No requirement of prior DNA extraction or any other intermediary steps leads to SHORT PCR protocol times.
  • Robust Reagents — Proprietary buffers help extract more DNA from any plant tissue. Our reagents are more tolerant to plant PCR inhibitors such as polysaccharides, polyphenols, and others,
  • Tested Reproducibility — Tested thoroughly for reproducible and accurate results.
  • Sensitive — High expression from each experiment
  • Convenient — Compatible with all real-time PCR instruments.

Intact Genomics FastAmp Plant Direct PCR kit is an easy and robust method to amplify DNA fragments from plant tissues with high specificity and high sensitivity without the need for complicated DNA purification steps.  The advanced formulation of this kit also allows fast PCR cycling conditions without compromising PCR sensitivity, specificity, and yield. The FastAmp Plant Direct PCR kit is an ideal and powerful tool for high-throughput genotyping, DNA amplification, and plant genome analysis.

FastAmp Plant Direct PCR Kit is suitable for amplification of DNA directly from plant samples without purifying DNA. This kit is based on specially engineered Taq DNA polymerase, proprietary buffer system, dNTP, MgCl2, PCR facilitators, and dye mix which makes it extremely robust and tolerant of plant PCR inhibitors such as complex polysaccharides, polyphenols, and others. This kit has been tested with leaves and seeds from a wide variety of plant species. FastAmp® Plant Direct PCR Kit includes a complete set of optimized reagents and detailed protocols making it an ideal choice for amplification of plant DNA without DNA purification.

Highlights

  • Direct PCR- no need to purify DNA
  • Specially engineered Taq DNA polymerase with the highest sensitivity and specificity
  • Extremely short PCR protocol times
  • Master mix format with premixed gel loading dye to reduce cross-contamination and sample handling
    errors
  • Optimized for both low and high GC templates

Applications

  • Genotyping
  • Transgene detection
  • Knockout analysis
  • Sequencing

 Product Includes

  • FastAmp Plant Direct PCR master mix (2x)
  • FastAmp Plant Direct PCR/Genotyping Solution

Storage Temperature 20ºC

Quality Control Assays

FastAmp Plant Direct PCR Kit has been tested with tissue from a wide variety of plant species, some of the results are included here. Direct PCR analysis from various 1.2mm punched leaf tissue and scratch tissue using FastAmp Plant Direct PCR kit.

 

 

Alejandra