chiptested

On-Chip Carba NP check for correct

On-chip Carba NP check for correct and excessive throughput detection of carbapenemase-producing Enterobacteriaceae.

Given the nice risk posed by scientific Carbapenemase-producing Enterobacteriaceae (CPE), its speedy and correct detection is extremely required to information an infection remedy and to forestall severe outbreaks. Whereas the Carba NP check (CNPt) is a well-liked and methodologically easy colorimetric assay for detecting carbapenemase exercise, its scientific utility is proscribed by low throughput, poor sensitivity and obscure coloration interpretation because of the lack of quantitative data on time- and dose-dependent coloration adjustments.

 

To deal with these limitations, we developed a multiplexed microfluidic gadget (1280 chambers) that is ready to routinely generate 16 bacterial focus gradients and makes use of inexperienced channel depth to extract quantitative coloration adjustments as a operate of time. Right here we examined the dynamic coloration altering profiles of three reference and eight scientific micro organism isolates.

 

The outcomes demonstrated linear correlations between coloration developments and time (inside 0-120 min) or bacterial doses (inside OD600 of 0.8-8.0), enabling correct and reproducible detection of CPE with over 105 fold improved sensitivity for an NDM-producer (LOD of two.4 × 103 cells) and over 104 fold improved sensitivity for an OXA-48-producer (LOD of seven.2 × 104 cells) as in comparison with the standard CNPt technique (LOD above 1.3 × 109 cells).

 

As the primary digital microfluidic platform for CPE, this on-chip CNPt in contrast favourably with the standard bench-based technique by considerably improved sensitivity, excessive throughput, low consumption and automatization, favouring its potential scientific functions for speedy screening of CPE with a low value. Moreover, the outcomes additionally revealed dynamic signature related to micro organism, which can maintain promise for a brand new method to review divergent bacterial responses to antibiotic remedies.

chiptested

chiptested

DNA Gyrase Assay Kit
TG1003 100 assays
EUR 375.6

DNA Damage Assay kit
BC149-20T 20T
EUR 150

DNA Damage Assay Kit
MBS480414-1Kit 1Kit
EUR 775

DNA Damage Assay Kit
MBS480414-5x1Kit 5x1Kit
EUR 3545

Helicase DNA Unwinding Assay Kit
DU050K 50 assays
EUR 467.15

ApoBrdU DNA Fragmentation Assay Kit
55R-1396 60 assays
EUR 887
Description: DNA Fragmentation Assay Kit for use in the research laboratory

ApoBrdU DNA Fragmentation Assay Kit
K2070-60 60 assays
EUR 638
Description: Detects DNA Fragmentation by FACS or FL.

ApoBrdU DNA Fragmentation Assay Kit
GWB-AXR224 60 assays Ask for price

ApoBrdU DNA Fragmentation Assay Kit
K401-60 each
EUR 927.6

ApoDIRECT DNA Fragmentation Assay Kit
55R-1397 50 assays
EUR 873
Description: DNA Fragmentation Assay Kit for use in the research laboratory

ApoDIRECT DNA Fragmentation Assay Kit
K2071-50 50 assays
EUR 782
Description: Detects DNA Fragmentation by FACS or FL.

ApoDIRECT DNA Fragmentation Assay Kit
GWB-AXR225 50 assays Ask for price

ApoDIRECT DNA Fragmentation Assay Kit
K402-50 each
EUR 952.8

DNA Quantification Assay Kit (Fluorometric)
K539-200 each
EUR 314.4

DNA Quantification Assay Kit (Fluorometric)
K539-2000 each
EUR 470.4

DNA Content（Cell Cycle）Assay kit
BC130-50T 50T
EUR 150

Human DNA Polymerase Gamma Assay Kit
DPG100K 100 assays
EUR 372.78
Description: This product includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA.

Human DNA Polymerase Alpha Assay Kit
HDPA100K 100 assays
EUR 278.4
Description: This product includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA. The kit does not include the enzyme.

ApoBrdU-IHC DNA Fragmentation Assay Kit
55R-1398 50 assays
EUR 913
Description: DNA Fragmentation Assay Kit for use in the research laboratory

ApoBrdU-IHC DNA Fragmentation Assay Kit
K2072-50 50 assays
EUR 658
Description: Detects DNA Fragmentation by two-color TUNEL.

ApoBrdU-IHC DNA Fragmentation Assay Kit
GWB-AXR226 50 assays Ask for price

ApoBrdU-IHC DNA Fragmentation Assay Kit
K403-50 each
EUR 952.8

EpiQuik In Situ DNA Damage Assay Kit 
P-6001
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  • 2x96 assays
  • 96 Assays

Human TDP1 Assay Kit (DNA/Top1 Complex)
TG1004-1A 100 assays
EUR 672

Human TDP1 Assay Kit (DNA/Top1 Complex)
TG1004-2A 250 assays
EUR 792

Topoisomerase I Assay Kit (62.5ug SC DNA)
TG1015-2 250 assays
EUR 496.8

DNA-Binding Fluorescence Interference Assay Kit
DBF100K 100 assays
EUR 221.78

Topoisomerase I Assay Kit (25ug SC DNA)
TG1015-1 100 assays
EUR 362.4

DNA Topoisomerase DNA decatenation Assay Kit (enzyme not included)
DDC020K 20 assays
EUR 184.03
Description: This product includes 150 ul of 10 x assay buffer (Buffer T4), 105 ul of 10 x concatenated DNA, 30 ul of 50 x ATP (10 mM),120 ul of 0.4 M EDTA, 160 ul of 20 x fluorescence dye and 20 spin columns for 20 DNA decatenation assays. The assay buffer is optimized for bacterial topoisomerase IV. Bacterial topoisomerase IV enzymes are provided separately.

NanoGram DNA Assay Reagent
NDA500 500 assays
EUR 187.8
Description: This product includes 5 ml of 10 x Reagent D. It is for 2000 assays using 384-well plates or 500 assays using 96-well plates. Cuvettes may also be used for the assay.

Human DNA Polymerase Beta Assay Kit Plus
DPB100KE 100 assays
EUR 448.28
Description: This product includes 0.800 ml of 10 x Buffer BP, 0.055 ml of 100x DNA template, 0.055 ml of 100 x dNTP mix, 0.055 ml of 100 x human DNA polymerase beta, 22 ml of Reagent U and 0.420 ml of 10 x fluorescence dye for 100 assays of human DNA polymerase reactions in a 96-well plate format.

Human DNA Polymerase Gamma Assay Kit Plus
DPG100KE 100 assays
EUR 467.15
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 33 ul of 100 x human DNA polymerase gamma, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA.

Human DNA Polymerase Alpha Assay Kit Plus
HDPA100KE1 100 assays
EUR 624.67
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP mix, 30 µl of 100 x human DNA polymerase alpha (4 µM), 1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.

Human DNA Polymerase Theta ATPase Assay Kit
HDPQ100K 100 assays
EUR 372.78

DNA polymerase Assay Kit (enzyme not included)
DPA100K 100 assays
EUR 278.4
Description: This product includes 500 ul of 10 x Buffer DP, 35 ul of 100 x DNA template, 35 ul of 100x dNTP mix and 320 ul of 10x fluorescence dye for 100 assays of DNA polymerase reactions in a 384-well assay format. The assay conditions are optimized for bacterial DNA polymerase III alpha subunit. Enzyme is not included in the kit.

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (500ug Relaxed DNA + 1000u Gyrase)
TG2000G-5KIT 1000 assays
EUR 1934.4

Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit
HCMV100K 100 assays
EUR 278.4
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP, 1550 ul of 2 x Dye and 1550 ul of 50 mM EDTA.

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (250ug Relaxed DNA + 500u Gyrase)
TG2000G-3KIT 500 assays
EUR 1182

Human Topoisomerase I DNA Relaxation Assay Kit -100
HRA100K 100 assays
EUR 460.55
Description: This product includes 480 ul of 10 x Buffer HT1, 405 ul of 10 x supercoiled plasmid DNA, 20 ul of 1500 x Dye H19 and 3000 ul of 10 x H19 dilution buffer. It is for 100 DNA relaxation assays in a 96-well plate format or 200 assays in a 384-well assay format. Human topo I enzyme in not included and can be purchased separately.

EpiQuik DNA Demethylase Activity/Inhibition Assay Kit 
P-3019
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  • Ask for price
  • 96 Assays
  • 48 Assays
  • 96 Assays

HIF-1 Alpha DNA Binding Activity Assay Kit
CBA-282 96 assays
EUR 762
Description: Cell Biolabs? HIF-1 Alpha DNA Binding Activity Assay Kit is an immunoassay developed for rapid detection of activated HIF-1 in any protein sample. Active HIF-1 complex is captured on a double stranded oligonucleotide containing an HRE that is attached to the well. HIF-1 Alpha is then detected with an anti-HIF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HIF-1 Alpha from human, mouse, or rat. The HIF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method.

ASF 2.0 DNA PCR Assay
TC-9094-064 each
EUR 800

Gel Shift Single-strand DNA Binding Assay Kit
GSSB100K 100 assays
EUR 112.31
Description: This product includes 150 µl of 10 x Buffer, 150 µl of 10 x ssDNA (a 45-mer single-strand DNA) and 100 µl of 4 x gel loading buffer.

Human DNA Polymerase Theta ATPase Assay Kit Plus
HDPQ100KE 100 assays
EUR 490.51

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (1mg Relaxed DNA + 2000u Gyrase)
TG2000G-7KIT 2000 assays
EUR 3412.8

DNA Topoisomerase I Assay Kit (enzyme not included)
DRA020K 20 assays
EUR 184.03
Description: This product includes the reaction buffer (T1 Buffer), supercoiled plasmid DNA and fluorescence dye H19 for assaying 20 DNA relaxation reactions in a 96-well plate assay format or 40 assays in a 384-well assay format. The reaction buffer is optimized for bacterial topoisomerase I.

Human DNA Polymerase Theta Helicase Assay Kit Plus
DU050PQ 50 assays
EUR 843.79

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (50ug Relaxed DNA + 100u Gyrase)
TG2000G-1KIT 100 assays
EUR 456

Uracil DNA Glycosylase (UNG) Magnetic Luminex Assay Kit
LKU605001-96T 96T
EUR 966

Thymine DNA Glycosylase (TDG) Magnetic Luminex Assay Kit
LKU603892-96T 96T
EUR 966

DNA Methyltransferase 1 (DNMT1) Magnetic Luminex Assay Kit
LKU606608-96T 96T
EUR 966

DNA Methyltransferase 1 (DNMT1) Magnetic Luminex Assay Kit
LKU606609-96T 96T
EUR 993.6

DNA Methyltransferase 1 (DNMT1) Magnetic Luminex Assay Kit
LKU606610-96T 96T
EUR 1048.8

E. coli DNA Helicase ATPase assay Kit Plus-100
DNAB100KE 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x E. coli DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

E. coli DNA Helicase ATPase assay Kit Plus-500
DNAB500KE 500 assays
EUR 1396.74
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x E. coli DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

DNA Methyltransferase 3A (DNMT3A) Magnetic Luminex Assay Kit
LKU606611-96T 96T
EUR 966

DNA Methyltransferase 3B (DNMT3B) Magnetic Luminex Assay Kit
LKU606612-96T 96T
EUR 993.6

DNA Methyltransferase 3B (DNMT3B) Magnetic Luminex Assay Kit
LKU606613-96T 96T
EUR 966

E. coli DNA Primase Assay Kit (enzyme not included)
EGA100K 100 assays
EUR 278.4
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of E. coli DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.

S. aureus DNA Helicase ATPase assay Kit Plus-100
DNAB100KS 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. aureus DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-500
DNAB500KS 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. aureus DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. aureus DNA Primase Assay Kit (enzyme not included)
AGA100K 100 assays
EUR 278.4
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of S. aureus DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.

S. aureus DNA Primase Assay Kit Plus (enzyme included)
AGA100KE 100 assays
EUR 444.91
Description: This product includes all the kit components in S. aureus DNA Primase Assay Kit (Catalog No. AGA100K ) plus 45 ul of 100 x S. aureus primase. It is for 100 assays of S. aureus DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.

Gel Shift Single-strand DNA Binding Assay Kit Plus
GSSB100KE 100 assays
EUR 332.56
Description: This product includes 150 µl of 10 x Buffer, 150 µl of 10 x ssDNA, 150 µl of 2 mg/ml E. coli single-strand DNA binding protein SSB and 100 µl of 4 x gel loading buffer.

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit 
P-3001
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  • Ask for price
  • 96 Assays
  • 48 Assays
  • 96 Assays

Topoisomerase I Assay Kit (125ug SC DNA + 500u Human Topo I)
TG1015-3A 500 assays
EUR 926.4

H. influenzae DNA Helicase ATPase assay Kit Plus-100
DNAB100KH 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x H. influenzae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-100
DNAB100KN 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. pneumoniae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-100
DNAB100KP 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x P. aeruginosa DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

H. influenzae DNA Helicase ATPase assay Kit Plus-500
DNAB500KH 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x H. influenzae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-500
DNAB500KN 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. pneumoniae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-500
DNAB500KP 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x P. aeruginosa DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. pneumonia DNA Primase Assay Kit (enzyme not included)
PGA100K 100 assays
EUR 278.4
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of S. pneumoniae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.

Human DNA polymerase beta assay kit (enzyme not included)
DPB100K 100 assays
EUR 278.4
Description: This product includes all the assay reagents except the enzyme for 100 assays of human DNA polymerase beta reactions in a 96-well plate format.

H. influenzae DNA Primase Assay Kit (enzyme not included)
HGA100K 100 assays
EUR 278.4
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of H. influenzae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.

Human Topoisomerase I DNA Relaxation Assay Kit Plus -1000
HRA1000KE 100 assays
EUR 3100.19
Description: This product includes 4800 ul of 10 x Buffer HT1, 4050 ul of 10 x supercoiled plasmid DNA, 40 ul of 1000 x human topoisomerase I enzyme (500U/ul), 170 ul of 1500 x Dye H19 and 30 ml of 10 x H19 dilution buffer. This product includes all the reagents for 1000 DNA relaxation assays in a 96-well plate format or 2000 assays in a 384-well assay format.

Human Topoisomerase I DNA Relaxation Assay Kit Plus -100
HRA100KE 100 assays
EUR 590.51
Description: This product includes 480 ul of 10 x Buffer HT1, 405 ul of 10 x supercoiled plasmid DNA, 10 ul of 1000 x human topoisomerase I enzyme (500U/ul), 20 ul of 1500 x Dye H19 and 3000 ul of 10 x H19 dilution buffer. This product includes all the reagents for 100 DNA relaxation assays in a 96-well plate format or 200 assays in a 384-well assay format.

S. pneumonia DNA Primase Assay Kit Plus (enzyme included)
PGA100KE 100 assays
EUR 444.91
Description: This product includes all the kit components in S. pneumoniae DNA Primase Assay Kit (Catalog No. PGA100K ) plus 45 ul of 100 x S. pneumoniae primase. It is for 100 assays of S. pneumoniae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.

H. influenzae DNA Primase Assay Kit Plus (enzyme included)
HGA100KE 100 assays
EUR 444.91
Description: This product includes all the kit components in H. influenzae DNA Primase Assay Kit (Catalog No. HGA100K ) plus 45 ul of 100 x H. influenzae primase-helicase complex. It is for 100 assays of H. influenzae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.

NAD-dependent DNA Ligase Assay Kit (enzyme not included)
NLA100K 100 assays
EUR 350.75
Description: This product includes 800 ul of 10 x buffer, 44 ul of 100x DNA substrate, 44 ul of 100 x NAD+, 21 ml of Reagent T and 220 ul of 10 x fluorescence dye for 100 assays. The kit does not include ligase that can be purchased separately (please see the information of related products).

DNA Topoisomerase II (Gyrase) Assay Kit (enzyme not included)
DSA020K 20 assays
EUR 184.03
Description: This product includes the reaction buffer (T2 Buffer), relaxed plasmid DNA and fluorescence dye H19 for 20 assays of DNA supercoiling reactions a 96-well plate assay format or 40 assays in a 384-well assay format.

DNA Repair Protein RAD50 (RAD50) Magnetic Luminex Assay Kit
LKU606619-96T 96T
EUR 993.6

DNA Repair Protein RAD50 (RAD50) Magnetic Luminex Assay Kit
LKU606620-96T 96T
EUR 966

H influenzae DNA Polymerase Assay Kit Plus (enzyme included)
DPA100KH 100 assays
EUR 444.91
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x H. influenzae DNA polymerase III alpha subunit.

S. pneumoniae DNA Polymerase Assay Kit Plus (enzyme included)
DPA100KN 100 assays
EUR 444.91
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x S. pneumoniae DNA polymerase III alpha subunit.

Gel-Based Human Topoisomerase I DNA relaxation Assay Kit
GHRA100KE 100 assays
EUR 414.97
Description: This product includes 400 ul of 10 x Buffer HT1, 100 ul of 0.25 mg/ml supercoiled DNA, 550 ul of 5 x Gel loading buffer and 10 ul of 500 U/ul human topoisomerase I enzyme.

EZClick? TUNEL ? in situ DNA Fragmentation/Apoptosis Assay Kit
K191-100 each
EUR 639.6

Polymerase DNA Directed Beta (POLb) Magnetic Luminex Assay Kit
LKU606621-96T 96T
EUR 966

Topoisomerase I Assay Kit (62.5ug SC DNA + 250u Human Topo I)
TG1015-2A 250 assays
EUR 792

Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit Plus-100
HCMV100KE 100 assays
EUR 444.91
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 33 ul of 100 x HCMV DNA polymerase, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA.

Topoisomerase I Assay Kit (25ug SC DNA +100u Human Topo I)
TG1015-1A 100 assays
EUR 483.6

N-Methylpurine DNA Glycosylase (MPG) Magnetic Luminex Assay Kit
LKU606861-96T 96T
EUR 966

GR DNA-Binding ELISA Kit
TFE-7082 1Kit
EUR 364
Description: GR TFactâ„¢ Colorimetric DNA-Binding ELISA Kit

Fos DNA-Binding ELISA Kit
TFE-7013 1Kit
EUR 364
Description: Fos TFactâ„¢ Colorimetric DNA-Binding ELISA Kit

ERF DNA-Binding ELISA Kit
TFE-7052 1Kit
EUR 364
Description: ERF TFactâ„¢ Colorimetric DNA-Binding ELISA Kit

A microfluidic chip-based co-culture of fibroblast-like synoviocytes with osteoblasts and osteoclasts to check bone erosion and drug analysis.

Focusing on fibroblast-like synoviocyte (FLS) migration and invasion-mediated bone erosion is a promising scientific technique for the remedy of rheumatoid arthritis (RA). Drug sensitivity testing is prime to this scheme. We designed a microfluidic chip-based, cell co-cultured platform to imitate RA FLS-mediated bone erosion and carry out drug-sensitive assay.

Human synovium SW982 cells had been cultured within the central channel and migrated to movement via matrigel-coated aspect channels in direction of cell tradition chamber the place RANKL-stimulated osteoclastic RAW264.7 and osteogenic medium (OS)-stimulated bone marrow mesenchymal stem cells (BMSC) had been cultured within the microfluidic chip gadget, mimicking FLS migration and invasion-mediated bone erosion in RA.

These SW982 cells confirmed completely different migration potentials to osteoclasts and BMSC. The migration of SW982 cells with excessive expression of cadherin-11 was stronger when SW982 cells had been linked with the co-culture of RAW264.7 and BMSC. Concurrently, within the co-cultured chamber, tartrate-resistant acid phosphatase (TRAP) exercise of RANKL-stimulated RAW264.7 cells was enhanced, however alkaline phosphatase (ALP) exercise was decreased compared with mono-cultured chamber.

 

Moreover, it was confirmed that celastrol, a constructive drug for the remedy of RA, inhibited SW982 cell migration in addition to TRAP exercise within the cell-cultured microfluidic chips. Thus, the migration and invasion to bone-related cells was reconstituted on the microfluidic mannequin. It might present an efficient anti-RA drug display mannequin for focusing on FLS migration-mediated bone erosion.

 

Altering the Blood Check: Correct Dedication of Mercury(II) in One Microliter of Blood Utilizing Oriented ZnO Nanobelt Array Movie Answer-Gated Transistor Chips.

The measurement of ultralow concentrations of heavy metallic ions (HMIs) in blood is difficult. A brand new technique for the dedication of mercury ions (Hg2+ ) primarily based on an oriented ZnO nanobelt (ZnO-NB) movie solution-gated field-effect transistor (FET) chip is adopted. The FET chips are fabricated with ZnO-NB movie channels with completely different orientations using the Langmuir-Blodgett (L-B) meeting approach.

 

The mixed simulation and I-V habits outcomes present that the nanodevice with ZnO-NBs parallel to the channel has distinctive efficiency. The sensing functionality of the oriented ZnO-NB movie FET chips corresponds to an ultralow minimal detectable degree (MDL) of 100 × 10-12 m in deionized water because of the change within the electrical double layer (EDL) arising from the synergism of the field-induced impact and the particular binding of Hg2+ to the thiol teams (-SH) on the movie floor. Furthermore, the ready FET chips current glorious selectivity towards Hg2+ , glorious repeatability, and a speedy response time (lower than 1 s) for varied Hg2+ concentrations. The sensing efficiency corresponds to a low MDL of 10 × 10-9 m in actual samples of a drop of blood.

 

 

Bovine Small Intestine, Ileum Frozen Sections
BF-309 10 slides
EUR 261

Guinea Pig Small Intestine, Ileum cDNA
GD-309 30 reactions
EUR 243

Bovine Small Intestine, Ileum Paraffin Sections
BP-309 10 slides
EUR 240

Monkey Small Intestine, Ileum cDNA, Rhesus
UD-309 30 reactions
EUR 316

Monkey Small Intestine, Ileum cDNA, Cynomolgus
KD-309 30 reactions
EUR 316

cDNA - Liver Cirrhosis: Small Intestine: Ileum
C1236227Lcs 40 reactions
EUR 802

Monkey Small Intestine, Ileum cDNA-Random Primer, Rhesus
UD-309-RH 30 reactions
EUR 316

Monkey Small Intestine, Ileum cDNA-Random Primer, Cynomolgus
KD-309-RH 30 reactions
EUR 316

cDNA - Human Adult Normal Tissue: Small Intestine: Ileum
C1234227 40 reactions
EUR 424

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum
C1534227 40 reactions
EUR 723

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Ileum
C1236227Dia 40 reactions
EUR 802

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Ileum
C1534227-Cy 40 reactions
EUR 723

Rat Intestine, Ileum cDNA
RD-309 30 reactions
EUR 243

Dog Small Intestine, Ileum Total RNA
DR-309 0.1mg
EUR 195

Cat Small Intestine, Ileum Total RNA
FR-309 0.1mg
EUR 195

Pig Small Intestine, Ileum Total RNA
PR-309 0.1mg
EUR 160

Sheep Small Intestine, Ileum Total RNA
SR-309 0.1mg
EUR 160

Rabbit Small Intestine, Ileum Total RNA
TR-309 0.1mg
EUR 160

Mouse CD1 Intestine, Ileum cDNA
MD-309 30 reactions
EUR 243

Mouse BLC Intestine, Ileum cDNA
MD-309-BLC 30 reactions
EUR 280

Mouse C57 Intestine, Ileum cDNA
MD-309-C57 30 reactions
EUR 280

Dog Small Intestine, Ileum Total Protein
DT-309 1mg
EUR 176

Hamster Small Intestine, Ileum Total RNA
AR-309 0.1mg
EUR 160

Cat Small Intestine, Ileum Total Protein
FT-309 1mg
EUR 176

Pig Small Intestine, Ileum Total Protein
PT-309 1mg
EUR 153

MiniPig Small Intestine, Ileum Total RNA
NR-309 0.1mg
EUR 231

Dog Small Intestine, Ileum Frozen Sections
DF-309 10 slides
EUR 261

Cat Small Intestine, Ileum Frozen Sections
FF-309 10 slides
EUR 261

Pig Small Intestine, Ileum Frozen Sections
PF-309 10 slides
EUR 261

Sheep Small Intestine, Ileum Total Protein
ST-309 1mg
EUR 153

Tissue cDNA, First Strand, Human Adult Normal, Small intestine, Ileum, BioGenomics
MBS651948-40Tests 40Tests
EUR 680

Tissue cDNA, First Strand, Human Adult Normal, Small intestine, Ileum, BioGenomics
MBS651948-5x40Tests 5x40Tests
EUR 2835

Rabbit Small Intestine, Ileum Total Protein
TT-309 1mg
EUR 153

Dog Small Intestine, Ileum paraffin Sections
DP-309 10 slides
EUR 240

Hamster Small Intestine, Ileum Total Protein
AT-309 1mg
EUR 153

Cat Small Intestine, Ileum Paraffin Sections
FP-309 10 slides
EUR 240

Human Small Intestine, Ileum Frozen Sections
HF-309 10slides
EUR 240

MiniPig Small Intestine, Ileum Total Protein
NT-309 1mg
EUR 176

Pig Small Intestine, Ileum Paraffin Sections
PP-309 10 slides
EUR 240

Sheep Small Intestine, Ileum Frozen Sections
SF-309 10 slides
EUR 261

Rabbit Small Intestine, Ileum Frozen Sections
TF-309 10 slides
EUR 240

Hamster Small Intestine, Ileum Frozen Sections
AF-309 10 slides
EUR 240

Human Small Intestine, Ileum Paraffin Sections
HP-309 10 slides
EUR 228

MiniPig Small Intestine, Ileum Frozen Sections
NF-309 10 slides
EUR 307

Sheep Small Intestine, Ileum Paraffin Sections
SP-309 10 slides
EUR 240

Bovine Small Intestine, Jejunum cDNA
BD-308 30 reactions
EUR 243

Hamster Small Intestine, Ileum Paraffin Sections
AP-309 10 slides
EUR 228

MiniPig Small Intestine, Ileum Paraffin Sections
NP-309 10 slides
EUR 307

Bovine Small Intestine, Duodenum cDNA
BD-307 30 reactions
EUR 243

Guinea Pig Small Intestine, Ileum Total RNA
GR-309 0.1mg
EUR 160

Monkey Small Intestine, Ileum Total RNA, Rhesus
UR-309 0.1mg
EUR 195

Guinea Pig Small Intestine, Ileum Total Protein
GT-309 1mg
EUR 153

Guinea Pig Small Intestine, Ileum Frozen Sections
GF-309 10 slides
EUR 240

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Small intestine, Ileum, BioGenomics
MBS652171-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Rhesus) Adult Normal, Small intestine, Ileum, BioGenomics
MBS652171-5x40Tests 5x40Tests
EUR 3600

Rat Intestine, Ileum cDNA-Random Primer
RD-309-RH 30 reactions
EUR 243

Monkey Small Intestine, Ileum Total RNA, Cynomolgus
KR-309 0.1mg
EUR 195

Mouse CD1 Small Intestine, Ileum Paraffin Sections
MP-309 10 slides
EUR 228

Mouse BLC Small Intestine, Ileum Paraffin Sections
MP-309-BLC 10 slides
EUR 253

Mouse C57 Small Intestine, Ileum Paraffin Sections
MP-309-C57 10 slides
EUR 253

Monkey Small Intestine, Ileum Total Protein, Rhesus
UT-309 1mg
EUR 176

Guinea Pig Small Intestine, Ileum Paraffin Sections
GP-309 10 slides
EUR 228

Tissue cDNA, First Strand, Human Diseased (Adult), Diabetes, Small Intestine, Ileum, BioGenomics
MBS652440-40Tests 40Tests
EUR 995

Tissue cDNA, First Strand, Human Diseased (Adult), Diabetes, Small Intestine, Ileum, BioGenomics
MBS652440-5x40Tests 5x40Tests
EUR 4250

Total Protein - Liver Cirrhosis: Small Intestine: Ileum
P1236227Lcs 1 mg
EUR 542

Monkey Small Intestine, Ileum Frozen Sections, Rhesus
UF-309 10 slides
EUR 307

Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Small intestine, Ileum, BioGenomics
MBS652396-40Tests 40Tests
EUR 995

Tissue cDNA, First Strand, Human Diseased, Liver Cirrhosis, Small intestine, Ileum, BioGenomics
MBS652396-5x40Tests 5x40Tests
EUR 4250

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Small Intestine, Ileum, BioGenomics
MBS651996-40Tests 40Tests
EUR 850

Tissue cDNA, First Strand, Monkey (Cynomolgus) Adult Normal, Small Intestine, Ileum, BioGenomics
MBS651996-5x40Tests 5x40Tests
EUR 3600

Monkey Small Intestine, Ileum Total Protein, Cynomolgus
KT-309 1mg
EUR 176

Monkey Small Intestine, Ileum Paraffin Sections, Rhesus
UP-309 10 slides
EUR 280

Rat WS Intestine, Ileum cDNA-Oligo-dT
RD-309-WS 30 reactions
EUR 243

Monkey Small Intestine, Ileum Frozen Sections, Cynomolgus
KF-309 10 slides
EUR 307

Monkey Small Intestine, Ileum Paraffin Sections, Cynomolgus
KP-309 10 slides
EUR 280

Mouse C57 Intestine, Ileum cDNA-Random Primer
MD-309-C57-RH 30 reactions
EUR 280

Mouse CD1 Intestine, Ileum cDNA-Random Primer
MD-309-HR 30 reactions
EUR 243

Total RNA - Human Adult Normal Tissue: Small Intestine: Ileum
R1234227-50 50 ug
EUR 221

Total RNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Ileum
R1534227-50 50 ug
EUR 229

Total Protein - Human Adult Normal Tissue: Small Intestine: Ileum
P1234227 1 mg
EUR 216

Tissue, Total Protein, Human Adult Normal, Small intestine, Ileum
MBS657599-1mg 1mg
EUR 565

Tissue, Total Protein, Human Adult Normal, Small intestine, Ileum
MBS657599-5x1mg 5x1mg
EUR 2315

Membrane Protein - Human Adult Normal Tissue: Small Intestine: Ileum
P3234227 0.1 mg
EUR 311

Tissue, Membrane Protein, Human Adult Normal, Small intestine, Ileum
MBS639932-01mg 0.1mg
EUR 570

Tissue, Membrane Protein, Human Adult Normal, Small intestine, Ileum
MBS639932-5x01mg 5x0.1mg
EUR 2345