chiptested

On-Chip Carba NP check for correct

On-chip Carba NP check for correct and excessive throughput detection of carbapenemase-producing Enterobacteriaceae.

Given the nice risk posed by scientific Carbapenemase-producing Enterobacteriaceae (CPE), its speedy and correct detection is extremely required to information an infection remedy and to forestall severe outbreaks. Whereas the Carba NP check (CNPt) is a well-liked and methodologically easy colorimetric assay for detecting carbapenemase exercise, its scientific utility is proscribed by low throughput, poor sensitivity and obscure coloration interpretation because of the lack of quantitative data on time- and dose-dependent coloration adjustments.

 

To deal with these limitations, we developed a multiplexed microfluidic gadget (1280 chambers) that is ready to routinely generate 16 bacterial focus gradients and makes use of inexperienced channel depth to extract quantitative coloration adjustments as a operate of time. Right here we examined the dynamic coloration altering profiles of three reference and eight scientific micro organism isolates.

 

The outcomes demonstrated linear correlations between coloration developments and time (inside 0-120 min) or bacterial doses (inside OD600 of 0.8-8.0), enabling correct and reproducible detection of CPE with over 105 fold improved sensitivity for an NDM-producer (LOD of two.4 × 103 cells) and over 104 fold improved sensitivity for an OXA-48-producer (LOD of seven.2 × 104 cells) as in comparison with the standard CNPt technique (LOD above 1.3 × 109 cells).

 

As the primary digital microfluidic platform for CPE, this on-chip CNPt in contrast favourably with the standard bench-based technique by considerably improved sensitivity, excessive throughput, low consumption and automatization, favouring its potential scientific functions for speedy screening of CPE with a low value. Moreover, the outcomes additionally revealed dynamic signature related to micro organism, which can maintain promise for a brand new method to review divergent bacterial responses to antibiotic remedies.

chiptested

chiptested

ApoBrdU DNA Fragmentation Assay Kit

K401-60 each
EUR 927.6

ApoDIRECT DNA Fragmentation Assay Kit

55R-1397 50 assays
EUR 1303.2
Description: DNA Fragmentation Assay Kit for use in the research laboratory

ApoDIRECT DNA Fragmentation Assay Kit

K2071-50 50 assays
EUR 919.2

ApoDIRECT DNA Fragmentation Assay Kit

K402-50 each
EUR 952.8

DNA Quantification Assay Kit (Fluorometric)

K539-200 each
EUR 314.4

DNA Quantification Assay Kit (Fluorometric)

K539-2000 each
EUR 470.4

Human DNA Polymerase Gamma Assay Kit

DPG100K 100 assays
EUR 372.78
Description: This product includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA.

Human DNA Polymerase Alpha Assay Kit

HDPA100K 100 assays
EUR 278.4
Description: This product includes all the assay kit components except the enzyme for 100 assays in a 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA. The kit does not include the enzyme.

ApoBrdU-IHC DNA Fragmentation Assay Kit

55R-1398 50 assays
EUR 1303.2
Description: DNA Fragmentation Assay Kit for use in the research laboratory

ApoBrdU-IHC DNA Fragmentation Assay Kit

K2072-50 50 assays
EUR 1002

ApoBrdU-IHC DNA Fragmentation Assay Kit

K403-50 each
EUR 952.8

DNA Topoisomerase DNA decatenation Assay Kit (enzyme not included)

DDC020K 20 assays
EUR 184.03
Description: This product includes 150 ul of 10 x assay buffer (Buffer T4), 105 ul of 10 x concatenated DNA, 30 ul of 50 x ATP (10 mM),120 ul of 0.4 M EDTA, 160 ul of 20 x fluorescence dye and 20 spin columns for 20 DNA decatenation assays. The assay buffer is optimized for bacterial topoisomerase IV. Bacterial topoisomerase IV enzymes are provided separately.

EpiQuik In Situ DNA Damage Assay Kit 

P-6001
  • EUR 1241.46
  • EUR 552.20
  • 2x96 assays
  • 96 Assays

Human TDP1 Assay Kit (DNA/Top1 Complex)

TG1004-1A 100 assays
EUR 672

Human TDP1 Assay Kit (DNA/Top1 Complex)

TG1004-2A 250 assays
EUR 792

Topoisomerase I Assay Kit (62.5ug SC DNA)

TG1015-2 250 assays
EUR 496.8

Topoisomerase I Assay Kit (25ug SC DNA)

TG1015-1 100 assays
EUR 362.4

NanoGram DNA Assay Reagent

NDA500 500 assays
EUR 187.8
Description: This product includes 5 ml of 10 x Reagent D. It is for 2000 assays using 384-well plates or 500 assays using 96-well plates. Cuvettes may also be used for the assay.

Human DNA Polymerase Beta Assay Kit Plus

DPB100KE 100 assays
EUR 448.28
Description: This product includes 0.800 ml of 10 x Buffer BP, 0.055 ml of 100x DNA template, 0.055 ml of 100 x dNTP mix, 0.055 ml of 100 x human DNA polymerase beta, 22 ml of Reagent U and 0.420 ml of 10 x fluorescence dye for 100 assays of human DNA polymerase reactions in a 96-well plate format.

Human DNA Polymerase Gamma Assay Kit Plus

DPG100KE 100 assays
EUR 467.15
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 33 ul of 100 x human DNA polymerase gamma, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA.

Human DNA Polymerase Alpha Assay Kit Plus

HDPA100KE1 100 assays
EUR 624.67
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 µl of 10 x Buffer, 33 µl of 100 x DNA template, 33 µl of 100 x dNTP mix, 30 µl of 100 x human DNA polymerase alpha (4 µM), 1550 µl of 2 x Dye, 1550 µl of 50 mM EDTA.

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (500ug Relaxed DNA + 1000u Gyrase)

TG2000G-5KIT 1000 assays
EUR 1934.4

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (250ug Relaxed DNA + 500u Gyrase)

TG2000G-3KIT 500 assays
EUR 1182

DNA polymerase Assay Kit (enzyme not included)

DPA100K 100 assays
EUR 278.4
Description: This product includes 500 ul of 10 x Buffer DP, 35 ul of 100 x DNA template, 35 ul of 100x dNTP mix and 320 ul of 10x fluorescence dye for 100 assays of DNA polymerase reactions in a 384-well assay format. The assay conditions are optimized for bacterial DNA polymerase III alpha subunit. Enzyme is not included in the kit.

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (1mg Relaxed DNA + 2000u Gyrase)

TG2000G-7KIT 2000 assays
EUR 3412.8

Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit

HCMV100K 100 assays
EUR 278.4
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP, 1550 ul of 2 x Dye and 1550 ul of 50 mM EDTA.

Human Topoisomerase I DNA Relaxation Assay Kit -100

HRA100K 100 assays
EUR 460.55
Description: This product includes 480 ul of 10 x Buffer HT1, 405 ul of 10 x supercoiled plasmid DNA, 20 ul of 1500 x Dye H19 and 3000 ul of 10 x H19 dilution buffer. It is for 100 DNA relaxation assays in a 96-well plate format or 200 assays in a 384-well assay format. Human topo I enzyme in not included and can be purchased separately.

Purified E. coli DNA Gyrase and Relaxed DNA Assay Kit (50ug Relaxed DNA + 100u Gyrase)

TG2000G-1KIT 100 assays
EUR 456

EpiQuik DNA Demethylase Activity/Inhibition Assay Kit 

P-3019
  • EUR 1067.46
  • EUR 477.40
  • EUR 710.60
  • 96 Assays
  • 48 Assays
  • 96 Assays

HIF-1 Alpha DNA Binding Activity Assay Kit

CBA-282 96 assays
EUR 762
Description: Cell Biolabs? HIF-1 Alpha DNA Binding Activity Assay Kit is an immunoassay developed for rapid detection of activated HIF-1 in any protein sample. Active HIF-1 complex is captured on a double stranded oligonucleotide containing an HRE that is attached to the well. HIF-1 Alpha is then detected with an anti-HIF-1 Alpha antibody followed by an HRP conjugated secondary antibody. Each kit provides sufficient reagents to perform up to a total of 96 assays, and can detect HIF-1 Alpha from human, mouse, or rat. The HIF-1 DNA Binding Activity Assay takes only 4.5 hours to complete compared to a standard 3 day electromobility shift (EMSA) method.

Gel Shift Single-strand DNA Binding Assay Kit

GSSB100K 100 assays
EUR 112.31
Description: This product includes 150 µl of 10 x Buffer, 150 µl of 10 x ssDNA (a 45-mer single-strand DNA) and 100 µl of 4 x gel loading buffer.

DNA Topoisomerase I Assay Kit (enzyme not included)

DRA020K 20 assays
EUR 184.03
Description: This product includes the reaction buffer (T1 Buffer), supercoiled plasmid DNA and fluorescence dye H19 for assaying 20 DNA relaxation reactions in a 96-well plate assay format or 40 assays in a 384-well assay format. The reaction buffer is optimized for bacterial topoisomerase I.

Uracil DNA Glycosylase (UNG) Magnetic Luminex Assay Kit

LKU605001-96T 96T
EUR 966

Thymine DNA Glycosylase (TDG) Magnetic Luminex Assay Kit

LKU603892-96T 96T
EUR 966

E. coli DNA Helicase ATPase assay Kit Plus-100

DNAB100KE 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x E. coli DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

E. coli DNA Helicase ATPase assay Kit Plus-500

DNAB500KE 500 assays
EUR 1396.74
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x E. coli DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

DNA Methyltransferase 1 (DNMT1) Magnetic Luminex Assay Kit

LKU606608-96T 96T
EUR 966

DNA Methyltransferase 1 (DNMT1) Magnetic Luminex Assay Kit

LKU606609-96T 96T
EUR 993.6

DNA Methyltransferase 1 (DNMT1) Magnetic Luminex Assay Kit

LKU606610-96T 96T
EUR 1048.8

E. coli DNA Primase Assay Kit (enzyme not included)

EGA100K 100 assays
EUR 278.4
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of E. coli DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.

DNA Methyltransferase 3A (DNMT3A) Magnetic Luminex Assay Kit

LKU606611-96T 96T
EUR 966

DNA Methyltransferase 3B (DNMT3B) Magnetic Luminex Assay Kit

LKU606612-96T 96T
EUR 993.6

DNA Methyltransferase 3B (DNMT3B) Magnetic Luminex Assay Kit

LKU606613-96T 96T
EUR 966

S. aureus DNA Helicase ATPase assay Kit Plus-100

DNAB100KS 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. aureus DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. aureus DNA Helicase ATPase assay Kit Plus-500

DNAB500KS 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. aureus DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. aureus DNA Primase Assay Kit (enzyme not included)

AGA100K 100 assays
EUR 278.4
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of S. aureus DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.

S. aureus DNA Primase Assay Kit Plus (enzyme included)

AGA100KE 100 assays
EUR 444.91
Description: This product includes all the kit components in S. aureus DNA Primase Assay Kit (Catalog No. AGA100K ) plus 45 ul of 100 x S. aureus primase. It is for 100 assays of S. aureus DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.

Gel Shift Single-strand DNA Binding Assay Kit Plus

GSSB100KE 100 assays
EUR 332.56
Description: This product includes 150 µl of 10 x Buffer, 150 µl of 10 x ssDNA, 150 µl of 2 mg/ml E. coli single-strand DNA binding protein SSB and 100 µl of 4 x gel loading buffer.

Topoisomerase I Assay Kit (125ug SC DNA + 500u Human Topo I)

TG1015-3A 500 assays
EUR 926.4

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit 

P-3001
  • EUR 1050.06
  • EUR 424.60
  • EUR 712.80
  • 96 Assays
  • 48 Assays
  • 96 Assays

H. influenzae DNA Helicase ATPase assay Kit Plus-100

DNAB100KH 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x H. influenzae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-100

DNAB100KN 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x S. pneumoniae DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-100

DNAB100KP 100 assays
EUR 444.91
Description: This product includes 0.500 ml of 10 x assay buffer, 0.035 ml of 100 x DNA, 0.030 ml of 100 x P. aeruginosa DNA helicase, 0.035 ml of 100 x ATP and 5 ml of dye for 100 assays in a 384-well assay format.

H. influenzae DNA Helicase ATPase assay Kit Plus-500

DNAB500KH 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x H. influenzae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. pneumoniae DNA Helicase ATPase assay Kit Plus-500

DNAB500KN 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x S. pneumoniae DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

P. aeruginosa DNA Helicase ATPase assay Kit Plus-500

DNAB500KP 500 assays
EUR 1491.11
Description: This product includes 2 ml of 10 x assay buffer, 0.170 ml of 100 x DNA, 0.150 ml of 100 x P. aeruginosa DNA helicase, 0.170 ml of 100 x ATP and 25 ml of dye for 500 assays in a 384-well assay format.

S. pneumonia DNA Primase Assay Kit (enzyme not included)

PGA100K 100 assays
EUR 278.4
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of S. pneumoniae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.

Human DNA polymerase beta assay kit (enzyme not included)

DPB100K 100 assays
EUR 278.4
Description: This product includes all the assay reagents except the enzyme for 100 assays of human DNA polymerase beta reactions in a 96-well plate format.

S. pneumonia DNA Primase Assay Kit Plus (enzyme included)

PGA100KE 100 assays
EUR 444.91
Description: This product includes all the kit components in S. pneumoniae DNA Primase Assay Kit (Catalog No. PGA100K ) plus 45 ul of 100 x S. pneumoniae primase. It is for 100 assays of S. pneumoniae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.

H. influenzae DNA Primase Assay Kit (enzyme not included)

HGA100K 100 assays
EUR 278.4
Description: This product includes 600 ul of 10 x assay buffer, 45 ul of 100 x DNA template, 45 ul of 100 x NTP mix and 850 ul of 10 x fluorescence dye for 100 assays of H. influenzae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format. The kit does not include the enzyme.

Human Topoisomerase I DNA Relaxation Assay Kit Plus -1000

HRA1000KE 100 assays
EUR 3100.19
Description: This product includes 4800 ul of 10 x Buffer HT1, 4050 ul of 10 x supercoiled plasmid DNA, 40 ul of 1000 x human topoisomerase I enzyme (500U/ul), 170 ul of 1500 x Dye H19 and 30 ml of 10 x H19 dilution buffer. This product includes all the reagents for 1000 DNA relaxation assays in a 96-well plate format or 2000 assays in a 384-well assay format.

Human Topoisomerase I DNA Relaxation Assay Kit Plus -100

HRA100KE 100 assays
EUR 590.51
Description: This product includes 480 ul of 10 x Buffer HT1, 405 ul of 10 x supercoiled plasmid DNA, 10 ul of 1000 x human topoisomerase I enzyme (500U/ul), 20 ul of 1500 x Dye H19 and 3000 ul of 10 x H19 dilution buffer. This product includes all the reagents for 100 DNA relaxation assays in a 96-well plate format or 200 assays in a 384-well assay format.

NAD-dependent DNA Ligase Assay Kit (enzyme not included)

NLA100K 100 assays
EUR 350.75
Description: This product includes 800 ul of 10 x buffer, 44 ul of 100x DNA substrate, 44 ul of 100 x NAD+, 21 ml of Reagent T and 220 ul of 10 x fluorescence dye for 100 assays. The kit does not include ligase that can be purchased separately (please see the information of related products).

H. influenzae DNA Primase Assay Kit Plus (enzyme included)

HGA100KE 100 assays
EUR 444.91
Description: This product includes all the kit components in H. influenzae DNA Primase Assay Kit (Catalog No. HGA100K ) plus 45 ul of 100 x H. influenzae primase-helicase complex. It is for 100 assays of H. influenzae DNA primase reactions in a 96-well plate format or 200 assays in 384-well assay format.

DNA Topoisomerase II (Gyrase) Assay Kit (enzyme not included)

DSA020K 20 assays
EUR 184.03
Description: This product includes the reaction buffer (T2 Buffer), relaxed plasmid DNA and fluorescence dye H19 for 20 assays of DNA supercoiling reactions a 96-well plate assay format or 40 assays in a 384-well assay format.

DNA Repair Protein RAD50 (RAD50) Magnetic Luminex Assay Kit

LKU606619-96T 96T
EUR 993.6

DNA Repair Protein RAD50 (RAD50) Magnetic Luminex Assay Kit

LKU606620-96T 96T
EUR 966

H influenzae DNA Polymerase Assay Kit Plus (enzyme included)

DPA100KH 100 assays
EUR 444.91
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x H. influenzae DNA polymerase III alpha subunit.

S. pneumoniae DNA Polymerase Assay Kit Plus (enzyme included)

DPA100KN 100 assays
EUR 444.91
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x S. pneumoniae DNA polymerase III alpha subunit.

Gel-Based Human Topoisomerase I DNA relaxation Assay Kit

GHRA100KE 100 assays
EUR 414.97
Description: This product includes 400 ul of 10 x Buffer HT1, 100 ul of 0.25 mg/ml supercoiled DNA, 550 ul of 5 x Gel loading buffer and 10 ul of 500 U/ul human topoisomerase I enzyme.

EZClick? TUNEL ? in situ DNA Fragmentation/Apoptosis Assay Kit

K191-100 each
EUR 639.6

Topoisomerase I Assay Kit (62.5ug SC DNA + 250u Human Topo I)

TG1015-2A 250 assays
EUR 792

Polymerase DNA Directed Beta (POLb) Magnetic Luminex Assay Kit

LKU606621-96T 96T
EUR 966

Topoisomerase I Assay Kit (25ug SC DNA +100u Human Topo I)

TG1015-1A 100 assays
EUR 483.6

Human Cytomegalovirus (HCMV) DNA Polymerase Assay Kit Plus-100

HCMV100KE 100 assays
EUR 444.91
Description: This product includes all the assay kit components for 100 assays in 384-well plate assay format: 400 ul of 10 x Buffer, 33 ul of 100 x DNA template, 33 ul of 100 x dNTP mix, 33 ul of 100 x HCMV DNA polymerase, 1550 ul of 2 x Dye, 1550 ul of 50 mM EDTA.

N-Methylpurine DNA Glycosylase (MPG) Magnetic Luminex Assay Kit

LKU606861-96T 96T
EUR 966

Tyrosyl DNA Phosphodiesterase 1 (TDP1) Magnetic Luminex Assay Kit

LKU607595-96T 96T
EUR 966

E. coli DNA polymerase Assay Kit Plus (E. coli DNA pol III alpha subunit included)

DPA100KE 100 assays
EUR 444.91
Description: This product includes all the reagents in the DNA Polymerase III Alpha Assay Kit (Catalog No. DPA100K) plus 35 ul of 100 x E. coli DNA polymerase III alpha subunit.

EpiQuik DNA Demethylase Activity/Inhibition Assay Ultra Kit 

P-3008
  • EUR 1032.66
  • EUR 454.30
  • EUR 686.40
  • 96 Assays
  • 48 Assays
  • 96 Assays

96-well E. coli Topo IV DNA Cleavage Assay Kit Plus

T4C96KE 96 assays
EUR 597.7
Description: This product includes all the components in TDC96K plus 26 ul of 100 x E. coli Topo IV.

Gel-Based E. coli Topoisomerase I DNA relaxation Assay Kit

GDRA100KE 100 assays
EUR 399.28
Description: This product includes all the reagents for 100 samples in gel-based assays of E. coli topo I DNA relaxation activity. It This product includes 300 µl of 10 x Buffer T1, 100 µl of 250 mg/ml supercoiled DNA, 550 µl of 5 x Gel loading buffer and 10 µl of 4 µM E. coli topoisomerase I enzyme.

Tar DNA Binding Protein 43kDa (TDP43) Magnetic Luminex Assay Kit

LKU601015-96T 96T
EUR 917.7

S. aureus Gyrase DNA Supercoiling Assay Kit Plus (enzyme included)

DSA020KSE 20 assays
EUR 240.65
Description: This product includes160 ul of 10 x Buffer T2, 84 ul of 10 x relaxed DNA, 6 ul of 1500 x H19 dye, 90 ul of 10 x ATP, 10 ul 7.5 uM S. aureus gyrase, 180 ul of 2 M potassium glutamate and 550 ul of 10 x H19 dilution buffer. It is for 20 assays in a 96-well plate format.

Polymerase DNA Directed Delta 1 (POLd) Magnetic Luminex Assay Kit

LKU606623-96T 96T
EUR 966

Polymerase DNA Directed Gamma 1 (POLg1) Magnetic Luminex Assay Kit

LKU606622-96T 96T
EUR 966

Gel-based Human Topoisomerase II DNA Decatenation Assay Kit Plus

HDD100KEG 100 assays
EUR 357.68
Description: This product includes 300 ul of 10 x assay buffer, 200 ul of 10 x concatenated DNA (30 ug/ml), 200 ul of 10 x ATP, 200 ul of 0.5 M EDTA, 15 ul of 200 x human topoisomerase II alpha (10 U/µl), 1 ml of enzyme dilution buffer and 500 ul of 5 x gel-loading buffer for 100 assays of human DNA decatenation reactions.

96-well Topoisomerase DNA Cleavage Assay Kit (enzyme not included)

TDC96K 96 assays
EUR 510.52
Description: This product includes 300 ul of 10 x reaction buffer, 30 ul of 10 x DNA, 260 ul of SDS solution, 30 ul of 100 mM ATP, 260 ul of proteinase K solutions, 10 ml of TDC matrix, 6 ml of rinse buffer, 500 ul of 10 x dye, a black receiver plate, a V-bottom reaction plate and a TDC filter plate. Topoisomerase is not included in the kit. A plate filtration device (see Note below) and a vacuum line are needed for the plate filtration process.

EpiQuik DNA Methyltransferase (DNMT) Activity/Inhibition Assay Kit (Fluorometric) 

P-3004
  • EUR 1067.46
  • EUR 429.00
  • EUR 710.60
  • 96 Assays
  • 48 Assays
  • 96 Assays

Human topoisomerase I DNA relaxation assay kit (enzyme not included)

HRA020K 20 assays
EUR 184.03
Description: This product includes 160 ul of 10 x Buffer HT1, 84 ul of 10 x supercoiled plasmid DNA, 8 ul of 1500 x Dye H19 and 500 ul of 10 x H19 dilution buffer. It is for 20 DNA relaxation assays in a 96-well plate format or 40 assays in a 384-well assay format. Human topo I enzyme in not included and can be purchased separately.

DNA Damage Inducible Transcript 3 (DDIT3) Magnetic Luminex Assay Kit

LKU606617-96T 96T
EUR 966

DNA Damage Inducible Transcript 3 (DDIT3) Magnetic Luminex Assay Kit

LKU606618-96T 96T
EUR 993.6

P. aeruginosa Gyrase DNA Supercoiling Assay Kit Plus (enzyme included)

DSA100KP 100 assays
EUR 590.51
Description: This product includes all the reagents for 100 assays of P. aeruginosa gyrase DNA supercoiling activity. This product includes 600 ul of 10 x Buffer T2, 405 ul of 10 x relaxed DNA, 20 ul of 1500 x H19 dye, 450 ul of 10 x ATP, 3000 ul of 10 x H19 dilution buffer and 50 ul 100 x P. aeruginosa gyrase.

E. coli Topo IV DNA Relaxation Assay Kit Plus (enzyme included)

T4RA-100KE 100 assays
EUR 561.75
Description: This product includes 480 ul of 10 x Buffer, 405 ul of 10 x supercoiled plasmid DNA, 45 ul of 100 x E. coli topoisomerase IV, 420 ul of 10 mM ATP and 20 ul of 1500 x fluorescence dye H19 for 100 assays of DNA relaxation reactions in a 96-well assay format.

Human Topoisomerase II DNA Decatenation Assay Kit (enzyme not included)

HDC020K 20 assays
EUR 184.03
Description: This product includes the assay buffer, concatenated DNA, spin columns and loading solution for 20 assays of DNA decatenation reactions. The assay buffer is optimized for human topoisomerase II alpha.

Human Topoisomerase II DNA Decatenation Assay Kit Plus (enzyme included)

HDC020KE 20 assays
EUR 240.65
Description: This product includes the assay buffer, concatenated DNA, human topoisomerase II alpha, spin columns and loading solution for 20 assays of DNA decatenation reactions. The assay buffer is optimized for human topoisomerase II alpha.

A microfluidic chip-based co-culture of fibroblast-like synoviocytes with osteoblasts and osteoclasts to check bone erosion and drug analysis.

Focusing on fibroblast-like synoviocyte (FLS) migration and invasion-mediated bone erosion is a promising scientific technique for the remedy of rheumatoid arthritis (RA). Drug sensitivity testing is prime to this scheme. We designed a microfluidic chip-based, cell co-cultured platform to imitate RA FLS-mediated bone erosion and carry out drug-sensitive assay.

Human synovium SW982 cells had been cultured within the central channel and migrated to movement via matrigel-coated aspect channels in direction of cell tradition chamber the place RANKL-stimulated osteoclastic RAW264.7 and osteogenic medium (OS)-stimulated bone marrow mesenchymal stem cells (BMSC) had been cultured within the microfluidic chip gadget, mimicking FLS migration and invasion-mediated bone erosion in RA.

These SW982 cells confirmed completely different migration potentials to osteoclasts and BMSC. The migration of SW982 cells with excessive expression of cadherin-11 was stronger when SW982 cells had been linked with the co-culture of RAW264.7 and BMSC. Concurrently, within the co-cultured chamber, tartrate-resistant acid phosphatase (TRAP) exercise of RANKL-stimulated RAW264.7 cells was enhanced, however alkaline phosphatase (ALP) exercise was decreased compared with mono-cultured chamber.

 

Moreover, it was confirmed that celastrol, a constructive drug for the remedy of RA, inhibited SW982 cell migration in addition to TRAP exercise within the cell-cultured microfluidic chips. Thus, the migration and invasion to bone-related cells was reconstituted on the microfluidic mannequin. It might present an efficient anti-RA drug display mannequin for focusing on FLS migration-mediated bone erosion.

 

Altering the Blood Check: Correct Dedication of Mercury(II) in One Microliter of Blood Utilizing Oriented ZnO Nanobelt Array Movie Answer-Gated Transistor Chips.

The measurement of ultralow concentrations of heavy metallic ions (HMIs) in blood is difficult. A brand new technique for the dedication of mercury ions (Hg2+ ) primarily based on an oriented ZnO nanobelt (ZnO-NB) movie solution-gated field-effect transistor (FET) chip is adopted. The FET chips are fabricated with ZnO-NB movie channels with completely different orientations using the Langmuir-Blodgett (L-B) meeting approach.

 

The mixed simulation and I-V habits outcomes present that the nanodevice with ZnO-NBs parallel to the channel has distinctive efficiency. The sensing functionality of the oriented ZnO-NB movie FET chips corresponds to an ultralow minimal detectable degree (MDL) of 100 × 10-12 m in deionized water because of the change within the electrical double layer (EDL) arising from the synergism of the field-induced impact and the particular binding of Hg2+ to the thiol teams (-SH) on the movie floor. Furthermore, the ready FET chips current glorious selectivity towards Hg2+ , glorious repeatability, and a speedy response time (lower than 1 s) for varied Hg2+ concentrations. The sensing efficiency corresponds to a low MDL of 10 × 10-9 m in actual samples of a drop of blood.

 

 

Total Protein - Human Diabetic Diseased Tissue: Small Intestine: Ileum

P1236227Dia 1 mg
EUR 471

Frozen Tissue Section - Human Diabetic Diseased Tissue: Small Intestine: Ileum

T1236227Dia 5 slides
EUR 475

Paraffin Tissue Section - Human Diabetic Diseased Tissue: Small Intestine: Ileum

T2236227Dia 5 slides
EUR 238

Innovative Grade US Origin Chicken Intestine Small Ileum Only

IGCHINTF each
EUR 133
Description: Innovative Grade US Origin Chicken Intestine Small Ileum Only

Innovative Grade US Origin Chicken Intestine Small Ileum Only

IGCHINTP each
EUR 210
Description: Innovative Grade US Origin Chicken Intestine Small Ileum Only

Innovative Grade US Origin Chicken Intestine Small Ileum Only

IGCHINTS each
EUR 210
Description: Innovative Grade US Origin Chicken Intestine Small Ileum Only

Innovative Grade US Origin Chicken Intestine Small Ileum Only

IGCHINTZ each
EUR 133
Description: Innovative Grade US Origin Chicken Intestine Small Ileum Only

Genomic DNA - Human Diabetic Diseased Tissue: Small Intestine: Ileum, from a single donor

D1236227Dia 50 ug
EUR 489

cDNA - Lupus: Small Intestine

C1236226Lup 40 reactions
EUR 737

cDNA - Liver Cirrhosis: Small Intestine

C1236226Lcs 40 reactions
EUR 729

cDNA - Rat Normal Tissue: Small Intestine

C1434226 40 reactions
EUR 578

cDNA - Dog Normal Tissue: Small Intestine

C1734226 40 reactions
EUR 385

cDNA - Mouse Normal Tissue: Small Intestine

C1334226 40 reactions
EUR 578

Innovative Grade US Origin Fetal Bovine Intestine Small

IGFBINTSF each
EUR 133
Description: Innovative Grade US Origin Fetal Bovine Intestine Small

Innovative Grade US Origin Fetal Bovine Intestine Small

IGFBINTSP each
EUR 210
Description: Innovative Grade US Origin Fetal Bovine Intestine Small

Innovative Grade US Origin Fetal Bovine Intestine Small

IGFBINTSS each
EUR 210
Description: Innovative Grade US Origin Fetal Bovine Intestine Small

Innovative Grade US Origin Fetal Bovine Intestine Small

IGFBINTSZ each
EUR 133
Description: Innovative Grade US Origin Fetal Bovine Intestine Small

cDNA - Liver Cirrhosis: Small Intestine: Jejunum

C1236230Lcs 40 reactions
EUR 729

cDNA - Liver Cirrhosis: Small Intestine: Duodenum

C1236101Lcs 40 reactions
EUR 729

cDNA - Human Adult Normal Tissue: Small Intestine

C1234226 40 reactions
EUR 385

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine

C1534226 40 reactions
EUR 578

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine

C1534226-Cy 40 reactions
EUR 578

cDNA - Human Adult Normal Tissue: Small Intestine: Jejunum

C1234230 40 reactions
EUR 385

cDNA - Human Adult Normal Tissue: Small Intestine: Duodenum

C1234101-10 10 reactions
EUR 215

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Jejunum

C1534230 40 reactions
EUR 578

cDNA - Monkey (Rhesus) Normal Tissue: Small Intestine: Duodenum

C1534101 40 reactions
EUR 578

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Jejunum

C1236230Dia 40 reactions
EUR 729

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Jejunum

C1534230-Cy 40 reactions
EUR 578

cDNA - Human Diabetic Diseased Tissue: Small Intestine: Duodenum

C1236101Dia 40 reactions
EUR 729

cDNA - Monkey (Cynomolgus) Normal Tissue: Small Intestine: Duodenum

C1534101-Cy 40 reactions
EUR 578

Small Intestine Lysate

1468 0.1 mg
EUR 229.2
Description: Small Intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small Intestine Lysate

1308 0.1 mg
EUR 229.2
Description: Small intestine tissue lysate was prepared by homogenization in modified RIPA buffer (150 mM sodium chloride, 50 mM Tris-HCl, pH 7.4, 1 mM ethylenediaminetetraacetic acid, 1 mM phenylmethylsulfonyl fluoride, 1% Triton X-100, 1% sodium deoxycholic acid, 0.1% sodium dodecylsulfate, 5 μg/ml of aprotinin, 5 μg/ml of leupeptin. Tissue and cell debris was removed by centrifugation. Protein concentration was determined with Bio-Rad protein assay. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small intestine cancer

SM208 each
EUR 546
Description: Small intestine cancer, metastatic lymph nodes and normal tissue high density tissue microarray, 69 cases/208 core, with stage and grade data

Small intestine disease spectrum (small intestine cancer progression) tissue array

SM802 each
EUR 306
Description: Small intestine disease spectrum (small intestine cancer progression) tissue array, with grade, stage and TNM data, 72 cases/80 cores

Small Intestine Tumor Lysate

1338 0.1 mg
EUR 304.8
Description: Small Intestine tumor tissue lysate was prepared by homogenization in lysis buffer (10 mM HEPES pH7.9, 1.5 mM MgCl2, 10 mM KCl, 1 mM ethylenediaminetetraacetic acid, 10% glycerol, 1% NP-40, and a cocktail of protease inhibitors). Tissue and cell debris was removed by centrifugation. The product was boiled for 5 min in 1 x SDS sample buffer (50 mM Tris-HCl pH 6.8, 12.5% glycerol, 1% sodium dodecylsulfate, 0.01% bromophenol blue) containing 50 mM DTT.

Small Intestine Membrane Lysate

XBL-10876 0.1 mg
EUR 619.8
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Small Intestine Cytoplasmic Lysate

XBL-10875 0.1 mg
EUR 273.3
Description: Human small intestine tissue cytoplasmic protein lysate was prepared by isolating the cytoplasmic protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The cytoplasmic protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, glycerol, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue cytoplasmic protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue cytoplasmic protein is then Western analyzed by GAPDH antibody, and the expression level is consistent with each lot.

Total RNA - Lupus: Small Intestine

R1236226Lup-50 50 ug
EUR 421.2

Rat Small Intestine Tissue Lysate

IRTINTSTL100UG each
EUR 245
Description: Rat Small Intestine Tissue Lysate

Human Small Intestine Tumor lysate

HTL-1338 1 mg
EUR 927.6

Small intestine Tissue Slide (Normal)

11-801-10um 10 um
EUR 241.8

Small intestine Tissue Slide (Normal)

11-801-4um 4 um
EUR 216.6

Normal small intestine tissue array

SMN601 each
EUR 222
Description: Normal small intestine tissue array, including TNM, clinical stage and pathology grade, 30 cases/ 60 cores

Mouse Small Intestine Tissue Lysate

IMSINTSTL100UG each
EUR 245
Description: Mouse Small Intestine Tissue Lysate

Small Intestine Tissue Slide (Abnormal)

11-824-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Abnormal)

11-824-4um 4 um
EUR 216.6

Small Intestine Membrane Tumor Lysate

XBL-10888 0.1 mg
EUR 752.1
Description: Human small intestine tissue membrane protein lysate was prepared by isolating the membrane protein from whole tissue homogenates using a proprietary technique. The human small intestine tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The membrane protein is provided in a buffer including HEPES (pH 7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the isolated small intestine tissue membrane protein pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The isolated small intestine tissue membrane protein is then Western analyzed by either GAPDH or β-actin antibody to confirm there is no signal or very weak signal.

Small Intestine Tissue Slide (Malignant)

11-814-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Malignant)

11-814-4um 4 um
EUR 216.6

Small Intestine Liver Cirrhosis Lysate

XBL-10377 0.1 mg
EUR 796.2
Description: Human small intestine tissue lysate was prepared by homogenization using a proprietary technique. The tissue was frozen in liquid nitrogen immediately after excision and then stored at -70°C. The human small intestine tissue total protein is provided in a buffer including HEPES (pH7.9), MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium deoxycholate, NP-40, and a cocktail of protease inhibitors. For quality control purposes, the small intestine tissue pattern on SDS-PAGE gel is shown to be consistent for each lot by visualization with coomassie blue staining. The small intestine tissue is then Western analyzed by either GAPDH or β-actin antibody, and the expression level is consistent with each lot.

Human Small Intestine Epithelial Cells

ABC-TC3814 1 vial Ask for price
Description: Human small intestines cells are derived from whole small intestines that have been dissociated into multi-cellular aggregates and single cells then frozen. Human whole small intestines cells are from a single donor. The small intestine consists of several specialized epithelial cells that could help researchers determine the absorption potential of drug candidates. In addition, these cells can be used with studies of transport and metabolism of drugs and dietary chemicals.

Human Small Intestine Endothelial Cells

HEC15 500,000+ Cells Frozen
EUR 1296

Human Small Intestine Endothelial Cells

ABC-TC3813 1 vial Ask for price
Description: Human Intestinal Microvascular Endothelial Cells were initiated by elutriation from dissociated normal human small intestine tissue.These cells were originated using Complete Serum-Free Medium Kit With AcceSup™, are available at <12 Cumulative Population Doublings (CPD) in vitro [Passage 3] and were cryopreserved in aliquots of ~1.5×10^6 cells. This vial will initiate a Passage 4 cell culture in a 75cm2 flask.

OCPB00059-100UG - Small Intestine Lysate

OCPB00059-100UG 0.1mg
EUR 139

OCPB00079-100UG - Small Intestine Lysate

OCPB00079-100UG 0.1mg
EUR 139

Small Intestine Tissue Slide (Adenocarcinoma)

11-815-10um 10 um
EUR 241.8

Small Intestine Tissue Slide (Adenocarcinoma)

11-815-4um 4 um
EUR 216.6

Rat Small Intestine Smooth Muscle Cells

ABC-TC4218 1 vial Ask for price
Description: Rat small intestine smooth muscle, 1.5-week Wistar rat

Rat Small Intestine Smooth Muscle Cells

CP-R043 5×105 cells/vial
EUR 580
Description: Rat

Rat Small Intestine Whole tissue lysate

RAL-1468 1 mg
EUR 628.8

Mouse Small Intestine Whole tissue lysate

MAL-1408 1 mg
EUR 628.8

Human Small Intestine Tumor Tissue Lysate

IHUINTSTLT100UG each
EUR 361
Description: Human Small Intestine Tumor Tissue Lysate

Mouse Small Intestine Smooth Muscle Cells

CP-M043 5×105 cells/vial
EUR 580
Description: Mouse

Total RNA - Liver Cirrhosis: Small Intestine

R1236226Lcs-50 50 ug
EUR 421.2

Small intestine carcinoma test tissue array

T421b each
EUR 48
Description: Small intestine carcinoma test tissue array, with normal small intestine tissues, including pathology grade, TNM and clinical stage, 6 cases/24 cores, replacing T421a

Total Protein - Liver Cirrhosis: Small Intestine

P1236226Lcs 1 mg
EUR 471

OCPB00113-100UG - Small Intestine Tumor Lysate

OCPB00113-100UG 0.1mg
EUR 199

Human Small Intestine Lysate Membrane Fraction

IHUINTSTLM100UG each
EUR 870
Description: Human Small Intestine Lysate Membrane Fraction

Total RNA - Rat Normal Tissue: Small Intestine

R1434226-50 50 ug
EUR 209

Mouse Small Intestine Tissue Lysate Lyophilized

IMSINTSTLLY200UG each
EUR 89
Description: Mouse Small Intestine Tissue Lysate Lyophilized

Total RNA - Human Tumor Tissue: Small Intestine

R1235226-10 10 ug
EUR 400

Genomic DNA - Rat Normal Tissue: Small Intestine

D1434226 100 ug
EUR 286

Total RNA - Mouse Normal Tissue: Small Intestine

R1334226-50 50 ug
EUR 209

OCPB00713-100UG - Small Intestine Membrane Lysate

OCPB00713-100UG 0.1mg
EUR 559

Human Small Intestine Lysate Cytoplasmic Fraction

IHUINTSTLCYT100UG each
EUR 393
Description: Human Small Intestine Lysate Cytoplasmic Fraction

Genomic DNA - Mouse Normal Tissue: Small Intestine

D1334226 100 ug
EUR 286

Total Protein - Human Tumor Tissue: Small Intestine

P1235226 1 mg
EUR 471

OCPB00712-100UG - Small Intestine Cytoplasmic Lysate

OCPB00712-100UG 0.1mg
EUR 279

Matched Pair Tissue Slides - Small Intestine Tissue

11-800-MPPT 1 pack
EUR 241.8
Description: The Matched Pair Paraffin Tissue (MPPT) slides are designed for identifying tumor-specific/metastasis genes or proteins. Slices from normal and malignant tissues are mounted on each MPPT slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across normal and cancerous tissue.

Total Protein - Mouse Normal Tissue: Small Intestine

P1334226 1 mg
EUR 188

Small intestine carcinoma and normal tissue array

SM801 each
EUR 306
Description: Small intestine carcinoma and normal tissue array, 77 cases/80 cores

Membrane Protein - Human Tumor Tissue: Small Intestine

P3235226 0.1 mg
EUR 410

Human Liver Cirrhosis Small Intestine Tissue Lysate

IHUINTSCIRTL100UG each
EUR 1413
Description: Human Liver Cirrhosis Small Intestine Tissue Lysate

Human Small Intestine Tumor Lysate Membrane Fraction

IHUINTSTLTM100UG each
EUR 1199
Description: Human Small Intestine Tumor Lysate Membrane Fraction

Multiple Species Tissue Slides - Small Intestine Tissue

11-800-MSTA 1 pack
EUR 241.8
Description: The Multiple Species Tissue Array (MSTA) slides were designed to study protein expression patterns in different cells and tissues from multiple species. Tissue slices from three different species are mounted on each MSTA slide which can then be treated as a single histological slide for H&E staining, immunohistochemistry, or in situ hybridization. This format allows a rapid analysis of protein expression and localization across different species. MSTA slides can also be used to quickly determine the species reactivity of a given antibody.